Comparison of 1-sided and 2 sided Michaelis-Menten transporters in a two compartment model without flow.
Model number: 0017
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Figure3: Determining Vmax and Km from initial velocity:
The quantity, VmaxEstB = V2*B2/t.max is plotted (Black Lines) against
B1+B2. The uppermost point of each nearly vertical line is an estimate of the velocity
of the transporter between 0 and 1 second.
VmaxEstB equals approximately
V2*dB2/dt = PSeffective*(B1-B2).
As the Michaelis-Menten transporter becomes saturated (increasing values of B1+B2 ranging over 6 orders of magnitude), the uppermost points of the vertical lines asymptotically approach Vmax (Green Line). Half of the value, Vmax/2 (Red line) intersects the upper outline of these vertical lines where B1+B2 = Km (dashed Blue line).
Two types of a saturable Michaelis-Menten transporter are considered
in this two compartment model without flow--a one sided transporter
for solute A and a two-sided transporter for solute B.
The model for solute A is cis- side driven. Concentration of A in V1, A1,
determines the fractional saturation, PSa/PSmax, where PSmax is Vmax/Km, and
PSa = PSmax/(1 + A1/Km).
The model for solute B is cis-trans driven. Concentration of B in both V1 and V2, B1 and B2 respectively, determine the fractional saturation, where PSmax is Vmax/Km and
PSb = PSmax/(1 + B1/Km + B2/Km).