# TranspMM.2sided.Comp2

Comparison of 1-sided and 2 sided Michaelis-Menten transporters in a two compartment model without flow.

Model number: 0017

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## Description

Figure3: Determining Vmax and Km from initial velocity:
The quantity, VmaxEstB = V2*B2/t.max is plotted (Black Lines) against
B1+B2. The uppermost point of each nearly vertical line is an estimate of the velocity
of the transporter between 0 and 1 second.
VmaxEstB equals approximately
V2*dB2/dt = PSeffective*(B1-B2).

As the Michaelis-Menten transporter
becomes saturated (increasing values of B1+B2 ranging over 6 orders of
magnitude), the uppermost points of the vertical lines
asymptotically approach Vmax (Green Line). Half
of the value, Vmax/2 (Red line) intersects the upper outline of
these vertical lines where B1+B2 = Km (dashed Blue line).

Two types of a saturable Michaelis-Menten transporter are considered
in this two compartment model without flow--a one sided transporter
for solute A and a two-sided transporter for solute B.
The model for solute A is cis- side driven. Concentration of A in V1, A1,
determines the fractional saturation, PSa/PSmax, where PSmax is Vmax/Km, and

PSa = PSmax/(1 + A1/Km).

The model for solute B is cis-trans driven. Concentration of B in both
V1 and V2, B1 and B2 respectively, determine the fractional saturation, where
PSmax is Vmax/Km and

PSb = PSmax/(1 + B1/Km + B2/Km).

## Equations

#### One sided Michaelis-Menten Transporter

#### Ordinary Differential Equations

#### Initial Conditions

#### Two sided Michaelis-Menten Transporter

#### Ordinary Differential Equations

#### Initial Conditions

The equations for this model may also be viewed by running the JSim model applet and clicking on the Source tab at the bottom left of JSim's Run Time graphical user interface. The equations are written in JSim's Mathematical Modeling Language (MML). See the Introduction to MML and the MML Reference Manual. Additional documentation for MML can be found by using the search option at the Physiome home page.

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## References

Klingenberg M. Membrane protein oligomeric structure and transport function. Nature 290: 449-454, 1981. Stein WD. The Movement of Molecules across Cell Membranes. New York: Academic Press, 1967. Stein WD. Transport and Diffusion across Cell Membranes. Orlando, Florida: Academic Press Inc., 1986. Wilbrandt W and Rosenberg T. The concept of carrier transport and its corollaries in pharmacology. Pharmacol Rev 13: 109-183, 1961. Schwartz LM, Bukowski TR, Ploger JD, and Bassingthwaighte JB. Endothelial adenosin transporter characterization in perfused guinea pig hearts. Am J Physiol Heart Circ Physiol 279: H1502-H1511, 2000.

## Related Models

Master Two Compartment Transporter Model (includes all cases):

Transporter models from Compartment Tutorial (mostly passive exchange):- Compartmental
- Comp2Exchange: 2 compartments, no flow, 1 solute, 2 sided passive transporter
- Comp2FlowExchange: 2 compartments, with flow, 1 solute, 2 sided passive transporter
- Comp2ExchangeReaction: 2 compartments, no flow, 2 solutes, 2 sided passive transporter
- Comp2FlowExchangeReaction: 2 compartments, with flow, 2 solutes, 2 sided passive transporter
- Comp2FlowMMExchangeReaction: 2 compartments, with flow, 2 solutes, 4 single 1 sided Michaelis-Menten transporters
- Distributed
- BTEX20: 2 distributed regions (PDE), with flow, 1 solute, 2 sided passive transporter
- CTEX20: 2 distributed regions (serially connected ODEs), with flow, 1 solute, 2 sided passive transporter
- CTEX20b: 2 distributed regions (serially connected ODEs), with flow, 1 solute, 2 sided passive and 1 sided Michaelis-Menten transporter

- No Flow
- TranspMM1sidedComp2: 2 compartments, no flow, 1 solute, 1 sided MM transporter
- TranspMM.2sided.Comp2: 2 compartments, no flow, 1 solute, both 1 sided and 2 sided MM transporters
- With Flow

- 1 solute
- Transp1sol.Comp2: 2 compartments, no flow, 1 solute, T1-T2 transporter
- Transp1sol.Comp2F: 2 compartments, with flow, 1 solute, T1-T2 transporter
- Two solutes
- Transp2sol.Comp2: 2 compartments, no flow, 2 solutes, competitive T1-T2 transporter
- Transp2sol.Comp2: 2 compartments, with flow, 2 solutes, competitive T1-T2 transporter
- Transp2sol.Distrib2F: 2 regions with flow, 2 solutes, both T1-T2 and passive transporters, Michaelis-Menten enzymatic reaction, Counter-Transport Faciliation

## Key Terms

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## Model History

Get Model history in CVS.## Acknowledgements

Please cite **www.physiome.org** in any publication for which this software is used and send one reprint to the address given below:

The National Simulation Resource, Director J. B. Bassingthwaighte, Department of Bioengineering, University of Washington, Seattle WA 98195-5061.

[This page was last modified 29Jun10, 10:01 am.]

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physiome.org provided by the following grants:** NIH/NIBIB BE08407 Software Integration,
JSim and SBW 6/1/09-5/31/13; NIH/NHLBI T15 HL88516-01 Modeling for Heart, Lung and Blood: From Cell to Organ,
4/1/07-3/31/11; NSF BES-0506477 Adaptive Multi-Scale Model Simulation,
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Transport and Exchange, 12/1/1980-11/30/01 and NIH/NIBIB R01 EB001973
JSim: A Simulation Analysis Platform, 3/1/02-2/28/07.